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The paper summarizes the current scholarship on how scholars in Jewish Istanbul from the mid-fifteenth century to early sixteenth-century—and specifically Karaite sages—viewed the legitimacy and aims of scientific studies. In addition, the paper discusses a hitherto unexplored testimony by Joseph Beghi, a Karaite scholar active in this milieu, about his own ideas regarding science. The paper demonstrates that Beghi was an enthusiastic advocate of the study of science. It further illustrates the profound influence his teachers as well as Karaite and Rabbanite works he read had on his attitudes towards science studies.