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Purpose. Measure immunization rates in subsidized child care and determine whether the population is representative of inner city preschool children. Methods . We identified 156 children, ages 0–60 months, in 14 inner-city child care centers enrolled in subsidized care and obtained demographic and immunization information. ZIP-code distribution of sample children was correlated with similarly aged children from the 2000 Census (family incomes .150% of poverty). Results. The overall subsidized child care population was geographically similar to all low-income children, ages 0–5, in Jacksonville (r.0.94). Only 73.3% were up-to-date at 3 months and 44.2% at 12 months of age (3 DTaP, 2 HIB, 2 IPV, 3 Hep B). Discussion. Our findings support the contention that children in subsidized child care are geographically representative of low-income, inner city preschool children. This study suggests that children enrolled in subsidized child care are both potentially underserved and an accessible window into the inner-city preschool population.