This investigation represented a continued partnership between Oregon Department of Fish and Wildlife and the City of Portland identifying fish distribution and abundance, and monitoring stream condition using an index of biotic integrity. From April 2008 through February 2009, we used backpack electrofishing techniques in the four City of Portland drainages Fanno Creek, Johnson Creek Watershed, Miller and Stephens creeks. Over 95% of the total aquatic animals identified in our catch were from three families, sculpin (family Cottidae; 55.2%), minnows (family Cyprinidae; 35.0%), and Pacific salmon and trout (family Salmonidae; 4.8%). Catch was predominated by sculpin during spring (64.8%) and summer (68.4%) while minnows predominated in fall (58.1%) and winter (47.1%). Salmon and trout were present every season with 3.5% to 5.1% spring through fall, and 9.7% during winter. Numbers of animals captured decreased spring to winter in three of the four drainages. Most of the fish captured were small (< 100 mm;< 3.9 inches fork length) in length. Sculpin appeared to use pooled and fast water hydrologic unit types (49% and 47%, respectively). Minnows preferred pools (82%) over the other two hydrologic unit types. Salmonids had a higher overall percentage capture in pools than fast water and glide habitats (66, 27, and 7%, respectively). Calculations of fish per estimated hydrologic units available in the entire reach contradicted the percentage findings for both sculpin and Pacific salmon and trout thus suggesting different pattern in distribution among hydrologic unit types, especially glides. On average, stream conditions in City of Portland were marginally impaired, and no reach achieved the highest ranking of acceptable impairment. Future monitoring in the region could be enhanced by focusing on assessing at a watershed level. Improving the power to detect slow recovery could require increasing the number of sites sampled, and by decreasing the duration between sampling events. A continued commitment to evaluate aquatic assemblages should inform our understanding of aquatic ecosystem condition in urban streams while assuring effective management of fish and wildlife in the region.