Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19
Y Okushima, PJ Overvoorde, K Arima, JM Alonso… - The Plant …, 2005 - academic.oup.com
Abstract The AUXIN RESPONSE FACTOR (ARF) gene family products, together with the
AUXIN/INDOLE-3-ACETIC ACID proteins, regulate auxin-mediated transcriptional
activation/repression. The biological function (s) of most ARFs is poorly understood. Here,
we report the identification and characterization of T-DNA insertion lines for 18 of the 23 ARF
gene family members in Arabidopsis thaliana. Most of the lines fail to show an obvious
growth phenotype except of the previously identified arf2/hss, arf3/ett, arf5/mp, and arf7/nph4 …
AUXIN/INDOLE-3-ACETIC ACID proteins, regulate auxin-mediated transcriptional
activation/repression. The biological function (s) of most ARFs is poorly understood. Here,
we report the identification and characterization of T-DNA insertion lines for 18 of the 23 ARF
gene family members in Arabidopsis thaliana. Most of the lines fail to show an obvious
growth phenotype except of the previously identified arf2/hss, arf3/ett, arf5/mp, and arf7/nph4 …
Methionine‐deficient diet extends mouse lifespan, slows immune and lens aging, alters glucose, T4, IGF‐I and insulin levels, and increases hepatocyte MIF levels and …
A diet deficient in the amino acid methionine has previously been shown to extend lifespan
in several stocks of inbred rats. We report here that a methionine‐deficient (Meth‐R) diet
also increases maximal lifespan in (BALB/cJ× C57BL/6 J) F1 mice. Compared with controls,
Meth‐R mice have significantly lower levels of serum IGF‐I, insulin, glucose and thyroid
hormone. Meth‐R mice also have higher levels of liver mRNA for MIF (macrophage
migration inhibition factor), known to be higher in several other mouse models of extended …
in several stocks of inbred rats. We report here that a methionine‐deficient (Meth‐R) diet
also increases maximal lifespan in (BALB/cJ× C57BL/6 J) F1 mice. Compared with controls,
Meth‐R mice have significantly lower levels of serum IGF‐I, insulin, glucose and thyroid
hormone. Meth‐R mice also have higher levels of liver mRNA for MIF (macrophage
migration inhibition factor), known to be higher in several other mouse models of extended …
[PDF][PDF] Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function
Apoptosis is initiated when Bcl-2 and its prosurvival relatives are engaged by proapoptotic
BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins.
These interactions have been considered promiscuous, but our analysis of the affinity of
eight BH3 peptides for five Bcl-2-like proteins has revealed that the interactions vary over
10,000-fold in affinity, and accordingly, only certain protein pairs associate inside cells. Bim
and Puma potently engaged all the prosurvival proteins comparably. Bad, however, bound …
BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins.
These interactions have been considered promiscuous, but our analysis of the affinity of
eight BH3 peptides for five Bcl-2-like proteins has revealed that the interactions vary over
10,000-fold in affinity, and accordingly, only certain protein pairs associate inside cells. Bim
and Puma potently engaged all the prosurvival proteins comparably. Bad, however, bound …