This article explores a midrash in the Babylonian Talmud that features the trope of the hind, an animal referred to multiple times in rabbinic literature as being noteworthy for its vaginal narrowness. The author argues that the midrash reveals significant awareness of Christian tropes about Mary the mother of Jesus—and in particular, tropes with especial currency in Christian circles in eastern Syria and the western reaches of the Sasanian Empire—and subtly subverts them. The effect of this deployment and subversion is a sustained polemic against the notion of Mary’s virginity, not only prior to, but even subsequent to the birth of Jesus. This case is then contextualized as part of a broader phenomenon of mariological critique in the Babylonian Talmud, a phenomenon that is in turn considered in light of the even broader historical trend in which Jewish communities develop awareness of and respond to increased Marian devotion among local Christian populations. This final point of comparison suggests that, in addition to polemic, we are likely to find examples of rabbinic appropriation of Marian themes in the Babylonian Talmud. One possible example is considered, but this remains a likely rich avenue for further research.